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CD3 antibody (PE)

CD3 Reactivity: Human, Mouse FACS, IP, IHC, WB, IF/ICC Host: Mouse Monoclonal PE

Catalog No. ABIN3037079

Datasheet as PDF

Target See all CD3 Antibodies

CD3
Reactivity
180

89

22

15

15

10

8

8

7

5

3

3

2

1
Human, Mouse
Host
205

45

14

13

9
Mouse
Compatible Secondaries
Clonality
275

7
Monoclonal
Conjugate
85

32

26

22

21

11

7

6

6

5

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD3 antibody is conjugated to PE
Application
258

78

53

44

41

35

27

23

9

8

7

6

4

3

2

2

2

2

2

2

2

1
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Specificity

The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Immunogen

Purified Human CD3 proteins isolated from thymus.

Isotype

IgG1

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CD3 Reactivity: Human FACS, IP, CyTOF Host: Mouse Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human FACS, IP, Func, CyTOF Host: Mouse Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF Host: Mouse Monoclonal UCHT1 unconjugated

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-57 Biotin

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro) Host: Mouse Monoclonal UCHT1 Biotin

anti-CD3 (CD3) antibody (APC)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 APC

anti-CD3 (CD3) antibody (PerCP)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PerCP

anti-CD3 (CD3) antibody (PE)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PE

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Buffer

The reagent is provided in stabilizing PBS solution containing 15  mM sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage Comment

Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Target

CD3

Alternative Name

CD3 (CD3 Products)

Synonyms

CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody

Gene ID

916

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway