GSTP1 antibody (AA 2-210)

Catalog No. ABIN3042437

Product Details anti-GSTP1 Antibody

Target: GSTP1 (Glutathione S-Transferase pi 1 (GSTP1))

Binding Specificity: AA 2-210

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSTP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Glutathione S-transferase P(GSTP1) detection. Tested with WB, IHC-P in Human,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Glutathione S-transferase P(GSTP1) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: glutathione S-transferase pi 1
Protein Name: Glutathione S-transferase P

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GST3 recombinant protein (Position: P2-Q210). Human GST3 shares 85% and 86% amino acid (aa) sequences identity with mouse and rat GST3, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for GST3 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-GSTP1 antibody (ABIN3042437)

Zhang, Yan, Xu, Fang, Zhang, Zhang, Wu, Kong, Huang: "The recovery trajectory of adolescent social defeat stress-induced behavioral, (1)H-MRS metabolites and myelin changes in Balb/c mice." in: Scientific reports, Vol. 6, pp. 27906, (2018) (PubMed).

Sun, Hu, Xiong, Tu, Zhao, Chen, Song, Ye: "Enhanced expression of stem cell markers and drug resistance in sphere-forming non-small cell lung cancer cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 6, pp. 6287-300, (2016) (PubMed).

Yang, Gao, Zhang, Fang, Wu, Xu, Huang: "Changes in proinflammatory cytokines and white matter in chronically stressed rats." in: Neuropsychiatric disease and treatment, Vol. 11, pp. 597-607, (2015) (PubMed).

Target Details for GSTP1

Target: GSTP1 (Glutathione S-Transferase pi 1 (GSTP1))

Alternative Name: GSTP1 (GSTP1 Products)

Synonyms: DFN7 antibody, FAEES3 antibody, GST3 antibody, GSTP antibody, PI antibody, GST-P antibody, Gst3 antibody, Gstp antibody, Gstp2 antibody, gst-Pi antibody, GstpiB antibody, glutathione S-transferase P-like antibody, glutathione S-transferase pi 1 antibody, glutathione S-transferase pi 1 L homeolog antibody, glutathione S-transferase, pi 1 antibody, LOC100739163 antibody, GSTP1 antibody, Gstp1 antibody, LOC100861197 antibody, gstp1.L antibody

Background: Glutathione S-transferases pi (GSTP1), also known as GST3, is an enzyme that in humans is encoded by the GSTP1 gene. This gene is mapped to 11q13.2. GSTP1 has 7 exons and 6 introns contained within approximately 2.8 kilobases. GSTP1 belongs to Glutathione S-transferases (GSTs) which are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 presents in all tissues and cells, with the exception of red cells, in which only erythrocyte GST(GSTe) is observed. What's more, GSTP1 is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

Synonyms: Deafness antibody|Deafness X-linked 7 antibody|DFN7 antibody|FAEES3 antibody|Fatty Acid Ethyl Ester Synthase III antibody|Glutathione S Transferase 3 antibody|Glutathione S Transferase Pi antibody|Glutathione S-transferase P antibody|Glutathione S-transferase pi 1 antibody|GST class-pi antibody|GST3 antibody|GSTP antibody|Gstp1 antibody|GSTP1-1 antibody|GSTP1_HUMAN antibody|PI antibody|X linked 7 antibody

Gene ID: 2950

UniProt: P09211

Pathways: Cellular Response to Molecule of Bacterial Origin