IL-1 beta antibody (AA 117-268)

Catalog No. ABIN3042509

Product Details anti-IL-1 beta Antibody

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Binding Specificity: AA 117-268

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit IgG polyclonal antibody for Interleukin-1 beta(IL1B) detection. Tested with WB, ELISA in Rat.

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Interleukin-1 beta(IL1B) detection. Tested with WB, ELISA in Rat.
Gene Name: interleukin 1, beta
Protein Name: Interleukin-1 beta(IL-1 beta)

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat IL-1 beta recombinant protein (Position: V117-S268). Rat IL-1 beta shares 78% and 90% amino acid (aa) sequences identity with human and mouse IL-1 beta, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, The detection limit for IL-1 beta is approximately 0.25 ng/lane under reducing conditions.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat

Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-IL-1 beta antibody (ABIN3042509)

Bi, Zeng, Zhao, Wei, Yu, Wang, Yu, Cao, Shan, Wei: "miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα." in: Molecular therapy. Nucleic acids, Vol. 5, Issue 9, pp. e368, (2016) (PubMed).

Wang, Gong, Chen, Xiong, Zhou, Huang, Kong: "NLRP3 inflammasome sequential changes in Staphylococcus aureus-induced mouse model of acute rhinosinusitis." in: International journal of molecular sciences, Vol. 15, Issue 9, pp. 15806-20, (2015) (PubMed).

Li, Chen, Zhang, Song, Mu: "Gastrodin inhibits neuroinflammation in rotenone-induced Parkinson's disease model rats." in: Neural regeneration research, Vol. 7, Issue 5, pp. 325-31, (2015) (PubMed).

Yu, Chen, Wang, Kuang, Liu, Zhang, Du: "Neuroprotective effect of kaempferol glycosides against brain injury and neuroinflammation by inhibiting the activation of NF-?B and STAT3 in transient focal stroke." in: PLoS ONE, Vol. 8, Issue 2, pp. e55839, (2013) (PubMed).

Yao, Peng, Peng, Tan, Wu, Wu, Chen, Li, Li, Zhu: "Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye." in: International journal of ophthalmology, Vol. 3, Issue 2, pp. 114-9, (2012) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1B (IL1B Products)

Synonyms: IL-1 antibody, IL1-BETA antibody, IL1F2 antibody, IL-1BETA antibody, IL1beta antibody, il1-b antibody, zgc:111873 antibody, IL-1B antibody, IL-1beta antibody, Il-1b antibody, IL1B antibody, IL-1 beta antibody, IL-1b antibody, interleukin 1 beta antibody, interleukin 1, beta antibody, IL1B antibody, il1b antibody, Il1b antibody

Background: Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Synonyms: Catabolin antibody|H1 antibody|Hematopoietin 1 antibody|IFN beta inducing factor antibody|IL 1 antibody|IL 1 beta antibody|IL 1B antibody|IL-1 beta antibody|IL1 BETA antibody|IL1B antibody|IL1B_HUMAN antibody|IL1F2 antibody|Interleukin 1 beta antibody|Interleukin 1 beta precursor antibody|Interleukin-1 beta antibody|LAF antibody|OAF antibody|Osteoclast activating factor antibody|OTTHUMP00000162031 antibody|Preinterleukin 1 beta antibody|Preinterleukin beta antibody|Pro interleukin 1 beta antibody

Gene ID: 24494

UniProt: Q63264

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome