NFKB1 antibody (C-Term)

Catalog No. ABIN3042654

There are 12+ publications for this product available. The Rabbit Polyclonal anti-NFKB1 antibody is suitable to detect NFKB1 in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for NFKB1 antibody (C-Term) (ABIN3042654)

Target: NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NFKB1 Antibody

Binding Specificity: AA 778-797, C-Term

Purpose: Anti-NFkB/NFKB1 p105/p50 Antibody

Sequence: WQVFDILNGK PYEPVFTSDD

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NFkB/NFKB1 p105/p50 Antibody (ABIN3042654). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse NFkB/NFKB1 p105/p50, identical to the related rat sequence, and different from the related human sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Thimerosal, 0.01 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-NFKB1 antibody (ABIN3042654)

Liu, Li, Liang, Li, Jiang, Chu, Yang: "Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway." in: International journal of molecular medicine, Vol. 41, Issue 4, pp. 1867-1876, (2018) (PubMed).

Wu, Gao, Li, Yao, Wang, Chen, Li, Ma, Ji, Li: "Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 2715718, (2017) (PubMed).

Zhao, Xue, Wang: "HMGB1, TGF-β and NF-κB are associated with chronic allograft nephropathy." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 6138-6146, (2017) (PubMed).

Kan, Zhou, Jin, Yang: "Effects of PDTC on NF-?B expression and apoptosis in rats with severe acute pancreatitis-associated lung injury." in: International journal of clinical and experimental medicine, Vol. 8, Issue 3, pp. 3258-70, (2015) (PubMed).

Xiao, Gang, Wang, Wang, Zhao, Xu, Liu: "Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy." in: Biochemical and biophysical research communications, Vol. 457, Issue 2, pp. 221-6, (2015) (PubMed).

Shang, Chen, Nie, Xu, Chen, Zeng, Zhou: "MiR199b suppresses expression of hypoxia-inducible factor 1α (HIF-1α) in prostate cancer cells." in: International journal of molecular sciences, Vol. 14, Issue 4, pp. 8422-36, (2015) (PubMed).

Xu, Wang, Wu, Wang: "Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor ?B and Toll-Like Receptor 4 Expression in Rats with Acute Necrotizing Pancreatitis." in: Gut and liver, Vol. 9, Issue 3, pp. 411-6, (2015) (PubMed).

Liu, Wang, Qian, Li: "Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis." in: International journal of molecular medicine, Vol. 34, Issue 6, pp. 1492-504, (2014) (PubMed).

Li, Qian, Ju, Wang: "Upregulation of Toll-like receptor 2 expression in colorectal cancer infected by human cytomegalovirus." in: Oncology letters, Vol. 9, Issue 1, pp. 365-370, (2014) (PubMed).

Zhao, Wang, Liu, Sun, Kazuhiro: "Protective effects of glutamine in a rat model of endotoxemia." in: Molecular medicine reports, Vol. 6, Issue 4, pp. 739-44, (2012) (PubMed).

Leng, Liu, Yang, Cui, Li, Zhu, Xiong, Zhang, Chen: "The apoptotic effect and associated signalling of HSP90 inhibitor 17-DMAG in hepatocellular carcinoma cells." in: Cell biology international, Vol. 36, Issue 10, pp. 893-9, (2012) (PubMed).

Li, Chen, Zhang, Zhu, Liu, Liang, Sun, Liao, Gong: "Protective effect of glutamine-enriched early enteral nutrition on intestinal mucosal barrier injury after liver transplantation in rats." in: American journal of surgery, Vol. 199, Issue 1, pp. 35-42, (2010) (PubMed).

Target Details for NFKB1

Target: NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))

Alternative Name: NFKB1

Background:

Synonyms: Nuclear factor NF-kappa-B p105 subunit,DNA-binding factor KBF1,EBP-1,NF-kappa-B1 p84/NF-kappa-B1 p98,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1,Nuclear factor NF-kappa-B p50 subunit,Nfkb1,

Tissue Specificity: Major component of lens fiber gap junctions.

Background: Nuclear factor NF-kappa-B p105 subunit, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing.

Sequence Similarities: Contains 7 ANK repeats.

Molecular Weight: 50, 120kDa

UniProt: P25799

Pathways: p53 Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins