TRAF1 antibody (Middle Region)

Catalog No. ABIN3042953

Product Details anti-TRAF1 Antibody

Target: TRAF1 (TNF Receptor-Associated Factor 1 (TRAF1))

Binding Specificity: AA 220-235, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAF1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for TNF receptor-associated factor 1(TRAF1) detection. Tested with WB in Human,Mouse.

Sequence: HQSQLDRERI LSLEQR

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for TNF receptor-associated factor 1(TRAF1) detection. Tested with WB in Human,Mouse.
Gene Name: TNF receptor-associated factor 1
Protein Name: TNF receptor-associated factor 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human TRAF1(220-235aa HQSQLDRERILSLEQR), different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, The detection limit for TRAF1 is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TRAF1

Target: TRAF1 (TNF Receptor-Associated Factor 1 (TRAF1))

Alternative Name: TRAF1 (TRAF1 Products)

Synonyms: CG3048 antibody, DTRAF antibody, DTRAF1 antibody, Dmel\\CG3048 antibody, TRAF antibody, TRAF1 antibody, Traf1 antibody, dTRAF antibody, dTRAF1 antibody, dTraf1 antibody, dtraf1 antibody, traf1 antibody, traf4 antibody, DKFZp469G032 antibody, EBI6 antibody, MGC:10353 antibody, 4732496E14Rik antibody, TNF receptor associated factor 1 antibody, TNF-receptor-associated factor 4 antibody, TNF receptor-associated factor 1 antibody, TRAF1 antibody, Traf4 antibody, Traf1 antibody, traf1 antibody

Background: TRAF1(TNF Receptor-Associated Factor 1), also called EBI6, is a protein that in humans is encoded by the TRAF1 gene. The protein encoded by this gene is a member of the TNF receptor(TNFR) associated factor(TRAF) protein family. Siemienski et al.(1997) used fluorescence in situ hybridization to map the TRAF1 gene to 9q33-q34. Mosialos et al.(1995) found that LMP1, the EBV-transforming protein, specifically associates with LAP1(TRAF3) or EBI6 in B lymphoblasts. LMP1 expression redirects LAP1 and EBI6 from scattered cytoplasmic structures to LMP1 plasma membrane patches. Both LAP1 and EBI6 associated with the cytoplasmic domain of p80/TNFR2 in vivo.

Synonyms: 4732496E14Rik antibody|EBI6 antibody|EBV induced protein 6 antibody|Epstein-Barr virus-induced protein 6 antibody|MGC:10353 antibody|Tnf receptor associated factor 1 antibody|TNF receptor-associated factor 1 antibody|TRAF 1 antibody|TRAF1 antibody|TRAF1_HUMAN antibody

UniProt: Q13077

Pathways: NF-kappaB Signaling, Apoptosis, Cell-Cell Junction Organization, Asymmetric Protein Localization