TJP3 antibody (C-Term)

Catalog No. ABIN3042974

Product Details anti-TJP3 Antibody

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Binding Specificity: AA 913-933, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Tight junction protein ZO-3(TJP3) detection. Tested with WB in Human.

Sequence: RVHDAESSDE DGYDWGPATD L

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Tight junction protein ZO-3(TJP3) detection. Tested with WB in Human.
Gene Name: tight junction protein 3
Protein Name: Tight junction protein ZO-3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Zonula occludens protein 3(913-933aa RVHDAESSDEDGYDWGPATDL).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TJP3

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Alternative Name: TJP3 (TJP3 Products)

Synonyms: cb766 antibody, wu:fj93c08 antibody, zo-3 antibody, zo3 antibody, ZO-3 antibody, ZO3 antibody, tight junction protein 3 antibody, tight junction protein 3 S homeolog antibody, tjp3 antibody, tjp3.S antibody, TJP3 antibody, Tjp3 antibody

Background: TJP3(TIGHT JUNCTION PROTEIN 3), also called ZO3, is a protein that in humans is encoded by the TJP3 gene. TJP3 is a member of the family of membrane-associated guanylate kinase-like proteins(MAGUK) that associate with intracellular junctions(Itoh et al., 1999). Haskins et al.(1998) showed that recombinant Tjp3 binds in vitro translated Tjp1 and binds directly to the cytoplasmic tail of occludin but no binding was detected between Tjp3 and Tjp2. Roh et al.(2002) determined that Tjp3 interacted with PATJ(INADL) and that both proteins colocalized to tight junctions in vivo. In vitro studies showed that the sixth and eighth PDZ domains of PATJ mediated interaction with the Tjp3 C-terminal domain and with Cldn1, respectively.

Synonyms: Tight junction protein 3 antibody|Tight junction protein ZO-3 antibody|Tight junction protein ZO3 antibody|TJP3 antibody|ZO3 antibody|ZO3_HUMAN antibody|Zona occludens protein 3 antibody|Zonula occludens protein 3 antibody

UniProt: O95049