ENO2/NSE antibody (C-Term)

Catalog No. ABIN3043022

Product Details anti-ENO2/NSE Antibody

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Binding Specificity: AA 420-434, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Gamma-enolase(ENO2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: EARFAGHNFR NPSVL

Cross-Reactivity (Details): Predicted Cross Reactivity: human, mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Gamma-enolase(ENO2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: enolase 2(gamma, neuronal)
Protein Name: Gamma-enolase

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NSE(420-434aa EARFAGHNFRNPSVL), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-ENO2/NSE antibody (ABIN3043022)

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Liu, Cheng, Wang, Zhan, Xiong, Zhao: "Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene." in: In vitro cellular & developmental biology. Animal, Vol. 51, Issue 3, pp. 319-27, (2015) (PubMed).

Zhang, Zhou, Zhou, Xu, Liao, Yan, Lv, Luo: "Methyl 3,4-dihydroxybenzoate promotes neurite outgrowth of cortical neurons cultured in vitro." in: Neural regeneration research, Vol. 7, Issue 13, pp. 971-7, (2015) (PubMed).

Zhang, Chai, Liu, Xu, Yang: "Synergistic effects of Buyang Huanwu decoction and embryonic neural stem cell transplantation on the recovery of neurological function in a rat model of spinal cord injury." in: Experimental and therapeutic medicine, Vol. 9, Issue 4, pp. 1141-1148, (2015) (PubMed).

Wang, Li, Qiu, Jiang, Simon, Ma, Liu, Liu, Wang, Liang, Wu, Di, Zhou: "Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons." in: PLoS ONE, Vol. 9, Issue 7, pp. e102161, (2015) (PubMed).

Wu, Chen, Guo, He, Hu: "Effects of transforming growth factor-?2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 8, pp. 4827-36, (2014) (PubMed).

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Liu, Teng, Cui, Wang, Liu, Zhang: "Amyloid beta-derived diffusible ligands (ADDLs) induce abnormal expression of insulin receptors in rat hippocampal neurons." in: Journal of molecular neuroscience : MN, Vol. 52, Issue 1, pp. 124-30, (2014) (PubMed).

Cai, Zhang, Wang, Wang, Liu, Guo, Yang, Wang, Wang, Shu, Xiao: "BDNF attenuates IL-1?-induced F-actin remodeling by inhibiting NF-?B signaling in hippocampal neurons." in: Neuro endocrinology letters, Vol. 35, Issue 1, pp. 13-9, (2014) (PubMed).

Hu, Xiang, Wen, He, Wu, Hu: "Neuroprotective effect of Atractylodes macrocephalaon polysaccharides in vitro on neuronal apoptosis induced by hypoxia." in: Molecular medicine reports, Vol. 9, Issue 6, pp. 2573-81, (2014) (PubMed).

Zhao, Yu, Wu, Liu, Zhou, Gu, Zhang, Zhang, Wu: "Anticancer activity of SAHA, a potent histone deacetylase inhibitor, in NCI-H460 human large-cell lung carcinoma cells in vitro and in vivo." in: International journal of oncology, Vol. 44, Issue 2, pp. 451-8, (2014) (PubMed).

Xu, Sun, Wu, Xi: "Investigation of characteristics of feather follicle stem cells and their regeneration potential." in: Journal of stem cells & regenerative medicine, Vol. 7, Issue 2, pp. 69-74, (2014) (PubMed).

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Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: ENO2 (ENO2 Products)

Synonyms: ENO2 antibody, DKFZp459B1817 antibody, NSE antibody, AI837106 antibody, D6Ertd375e antibody, Eno-2 antibody, RNEN3 antibody, eno3 antibody, wu:fc09h05 antibody, zgc:92418 antibody, enolase 2 antibody, enolase 2 (gamma, neuronal) antibody, enolase 2, gamma neuronal antibody, enolase 2, gamma, neuronal antibody, ENO2 antibody, Eno2 antibody, eno2 antibody

Background: NSE(neuron specific enolase), also known as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.

Synonyms: 2 phospho D glycerate hydrolyase antibody|2-phospho-D-glycerate hydro-lyase antibody|Eno 2 antibody|Eno2 antibody|ENOG antibody|ENOG_HUMAN antibody|Enolase 2 antibody|Enolase 2 gamma neuronal antibody|Enolase2 antibody|Gamma enolase antibody|Gamma-enolase antibody|Neural enolase antibody|Neuron specific enolase antibody|Neuron specific gamma enolase antibody|Neuron-specific enolase antibody|Neurone specific enolase antibody|NSE antibody

UniProt: P09104