Caspase 7 antibody (Middle Region)

Catalog No. ABIN3043217

Product Details anti-Caspase 7 Antibody

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Binding Specificity: AA 237-255, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Caspase-7(CASP7) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: RGSWFVQALC SILEEHGKD

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Caspase-7(CASP7) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: caspase 7, apoptosis-related cysteine peptidase
Protein Name: Caspase-7(CASP-7)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Caspase-7(P11)(237-255aa RGSWFVQALCSILEEHGKD), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Caspase 7

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Alternative Name: CASP7 (CASP7 Products)

Synonyms: xCaspase-7 antibody, caspase-7 antibody, caspase7 antibody, cmh-1 antibody, ice-lap3 antibody, mch3 antibody, CASP7 antibody, AI314680 antibody, CMH-1 antibody, ICE-IAP3 antibody, Mch3 antibody, mCASP-7 antibody, CASP-7 antibody, ICE-LAP3 antibody, LICE2 antibody, MCH3 antibody, Caspase-7 antibody, MCH3/SCA-2 antibody, SCA-2 antibody, caspase 7 antibody, caspase-7 antibody, caspase 7 S homeolog antibody, CASP7 antibody, xcaspase-7 antibody, casp7 antibody, casp7.S antibody, Casp7 antibody

Background: CASP7, Caspase-7, apoptosis-related cysteine peptidase, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. CASP7 is a member of the caspase(cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the CASP7 gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta(PRKCD)-dependent pathway.

Synonyms: Apoptosis related cysteine peptidase antibody|Apoptosis Related Cysteine Protease antibody|Apoptotic protease Mch-3 antibody|Apoptotic Protease MCH3 antibody|CASP 7 antibody|CASP-7 antibody|CASP7 antibody|Caspase-7 subunit p11 antibody|Caspase7 antibody|CMH 1 antibody|CMH-1 antibody|CMH1 antibody|ICE LAP3 antibody|ICE like Apoptotic Protease 3 antibody|ICE-LAP3 antibody|ICE-like apoptotic protease 3 antibody|MCH3 antibody

UniProt: P55210

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity