BRAF antibody (AA 38-230)

Catalog No. ABIN3043761

This anti-BRAF antibody is a Rabbit Polyclonal antibody detecting BRAF in WB, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for BRAF antibody (AA 38-230) (ABIN3043761)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-BRAF Antibody

Binding Specificity: AA 38-230

Purpose: Anti-B Raf/BRAF Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-B Raf/BRAF Antibody Picoband® (ABIN3043761). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Sithanandam G, Kolch W, Duh FM, Rapp UR (December 1990). "Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies". Oncogene 5 (12): 1775-80. 2. Sithanandam G, Druck T, Cannizzaro LA, Leuzzi G, Huebner K, Rapp UR (April 1992). "B-raf and a B-raf pseudogene are located on 7q in man". Oncogene 7 (4): 795-9. 3. Sithanandam, G., Kolch. W., Duh, F.-M., Rapp, U. R.Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies.Oncogene 5: 1775-1780, 1990.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF

Background:

Synonyms: Serine/threonine-protein kinase B-raf,2.7.11.1,Proto-oncogene B-Raf,p94,v-Raf murine sarcoma viral oncogene homolog B1,BRAF,BRAF1, RAFB1,

Tissue Specificity: Brain and testis.

Background: BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in ing cell growth.

Sequence Similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

Molecular Weight: 84 kDa

Gene ID: 673

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy