E-cadherin antibody (AA 286-703)

Catalog No. ABIN3043808

There are 30+ publications for this product available. The Rabbit Polyclonal anti-E-cadherin antibody is suitable to detect E-cadherin in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, ELISA, ICC and IHC (fro).

Quick Overview for E-cadherin antibody (AA 286-703) (ABIN3043808)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-E-cadherin Antibody

Binding Specificity: AA 286-703

Purpose: Anti-E Cadherin 1/CDH1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-E Cadherin 1/CDH1 Antibody (ABIN3043808). Tested in ELISA, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat By Heat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, Mouse, Rat -
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-E-cadherin antibody (ABIN3043808)

Liu, Li, Li, Teng, Wang, Zhang, Xiao: "TGF-β1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21." in: Scientific reports, Vol. 6, pp. 32231, (2018) (PubMed).

Zhang, Yao, Luan, Zhao, Jing, Zhang, Hou, Zou: "APC/CCDC20 and APC/C play pivotal roles in the process of embryonic development in Artemia sinica." in: Scientific reports, Vol. 6, pp. 39047, (2018) (PubMed).

Zhang, Bu, Sun, Xu, Yao, He, Lai: "Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 270, (2018) (PubMed).

Huang, Zheng, Pan, Wu, Su, Li, Wu, Sun: "Oral exposure of low-dose bisphenol A promotes proliferation of dorsolateral prostate and induces epithelial-mesenchymal transition in aged rats." in: Scientific reports, Vol. 8, Issue 1, pp. 490, (2018) (PubMed).

Wan, Tian, Qi, Liu, Xu: "ANXA1 affects cell proliferation, invasion and epithelial-mesenchymal transition of oral squamous cell carcinoma." in: Experimental and therapeutic medicine, Vol. 14, Issue 5, pp. 5214-5218, (2017) (PubMed).

Wang, An, Yu, Zhang, Tang, Zhang, Li: "MiR-29b/TET1/ZEB2 signaling axis regulates metastatic properties and epithelial-mesenchymal transition in breast cancer cells." in: Oncotarget, Vol. 8, Issue 60, pp. 102119-102133, (2017) (PubMed).

Lei, Lai, Bai, Qiu, Yang, Liao, Chuong, Yang, Lian, Zhong: "Prolonged overexpression of Wnt10b induces epidermal keratinocyte transformation through activating EGF pathway." in: Histochemistry and cell biology, Vol. 144, Issue 3, pp. 209-21, (2016) (PubMed).

Han, Wang, Huang, Cheng, Sun, Chen, Xie, Zhou, Du: "Isolation and characteristics of CD133‑/A2B5+ and CD133‑/A2B5‑ cells from the SHG139s cell line." in: Molecular medicine reports, Vol. 12, Issue 6, pp. 7949-56, (2016) (PubMed).

Tang, Gao, Mao, He, Liu, Cai, Lin, Wu: "Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways." in: Cell stress & chaperones, Vol. 21, Issue 2, pp. 239-49, (2016) (PubMed).

Ping, Liu, Liu, Li, Zhai, Li, Liu, Li, Wei: "Expression of RKIP, E-cadherin and NF-kB p65 in esophageal squamous cell carcinoma and their correlations." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10164-70, (2016) (PubMed).

Shan, Lv, Zhao, Liu, Sun, Xi, Xiao, Li: "Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 10, pp. 12357-67, (2016) (PubMed).

Wang, Chen, Liu, Qu, Lan, Chen, Zou, Qiu: "Regulator of cullins-1 expression knockdown suppresses the malignant progression of muscle-invasive transitional cell carcinoma by regulating mTOR/DEPTOR pathway." in: British journal of cancer, Vol. 114, Issue 3, pp. 305-13, (2016) (PubMed).

Lin, Sun, Wang, Zheng, Zhang, Zheng: "Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition." in: Frontiers in pharmacology, Vol. 6, pp. 320, (2016) (PubMed).

Cheng, Gu, Yuan, Feng, Jia: "Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-α/ERK1/2 pathway: An in vitro investigation." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3709-10, (2016) (PubMed).

Ge, Cao, Wang, Sun, Sun, Han, Cui, Liu: "Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-κB activation." in: OncoTargets and therapy, Vol. 9, pp. 1011-21, (2016) (PubMed).

Li, Chen, Zeng, Li, Zhang, Lin, Zhang, Xie, He, Shu, Yang, Tang, Fu: "Matrigel basement membrane matrix induces eccrine sweat gland cells to reconstitute sweat gland-like structures in nude mice." in: Experimental cell research, Vol. 332, Issue 1, pp. 67-77, (2015) (PubMed).

Deng, Zhang, Zhang, Wen, Miao, Guo: "MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7." in: Tumour biology, (2015) (PubMed).

Su, Li: "RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10760-7, (2015) (PubMed).

He, Qian, Cai, Yang, Cai, Wang: "RhoC is essential in TGF-β1 induced epithelial-mesenchymal transition in cervical cancer cells." in: Oncology letters, Vol. 10, Issue 2, pp. 985-989, (2015) (PubMed).

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Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1

Background:

Synonyms: Cadherin-1,CAM 120/80,Epithelial cadherin,E-cadherin,Uvomorulin,CD324,E-Cad/CTF1,E-Cad/CTF2,E-Cad/CTF3,CDH1,CDHE, UVO,

Tissue Specificity: Non-neural epithelial tissues.

Background: CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter). Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Sequence Similarities: Contains 5 cadherin domains.

Molecular Weight: 130 kDa

Gene ID: 999

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation