RAB5 antibody (Middle Region)
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- Target See all RAB5 (RAB5A) Antibodies
- RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))
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Binding Specificity
- AA 138-155, Middle Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RAB5 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Ras-related protein Rab-5A(RAB5A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Sequence
- ANKRAVDFQE AQSYADDN
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Ras-related protein Rab-5A(RAB5A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: RAB5A, member RAS oncogene family
Protein Name: Ras-related protein Rab-5A - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of human Rab5(138-155aa ANKRAVDFQEAQSYADDN), identical to the related rat and mouse sequences.
- Isotype
- IgG
- Top Product
- Discover our top product RAB5A Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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Change of Rin1 and Stathmin in the Animal Model of Traumatic Stresses." in: Frontiers in behavioral neuroscience, Vol. 11, pp. 62, (2017) (PubMed).
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Change of Rin1 and Stathmin in the Animal Model of Traumatic Stresses." in: Frontiers in behavioral neuroscience, Vol. 11, pp. 62, (2017) (PubMed).
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- Target
- RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))
- Alternative Name
- RAB5A (RAB5A Products)
- Synonyms
- RAB5 antibody, 2410015H04Rik antibody, AI663973 antibody, AU021172 antibody, nnyRab5a antibody, DDBDRAFT_0168594 antibody, DDBDRAFT_0229401 antibody, DDB_0168594 antibody, DDB_0229401 antibody, rab5 antibody, RAB5A antibody, Rab5a antibody, rab5al antibody, zgc:56644 antibody, fj38g08 antibody, hm:zehn1144 antibody, rab5a antibody, wu:fj38g08 antibody, RAB5A, member RAS oncogene family antibody, rab5A protein antibody, Rab5a, GTPase antibody, predicted protein antibody, Rab GTPase antibody, RAB5A, member RAS oncogene family L homeolog antibody, RAB5A, member RAS oncogene family, b antibody, RAB5A, member RAS oncogene family, a antibody, RAB5A antibody, Rab5a antibody, rab5A antibody, rab5a antibody, rab5a.L antibody, rab5ab antibody, rab5aa antibody
- Background
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RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP). RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.
Synonyms: RAB 5 antibody|RAB 5A antibody|RAB5A antibody|RAB5A member RAS oncogene family antibody|RAB5A_HUMAN antibody|RAS associated protein RAB5A antibody|Ras related protein Rab 5A antibody|Ras-related protein Rab-5A antibody - UniProt
- P20339
- Pathways
- Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
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