PIK3R2 antibody (Middle Region)

Catalog No. ABIN3044260

Product Details anti-PIK3R2 Antibody

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Binding Specificity: AA 447-461, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Phosphatidylinositol 3-kinase regulatory subunit beta(PIK3R2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: KVYHQQYQDK SREYD

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Phosphatidylinositol 3-kinase regulatory subunit beta(PIK3R2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: phosphoinositide-3-kinase, regulatory subunit 2(beta)
Protein Name: Phosphatidylinositol 3-kinase regulatory subunit beta(PI3-kinase regulatory subunit beta/PI3K regulatory subunit beta/PtdIns-3-kinase regulatory subunit beta)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PIK3R2(447-461aa KVYHQQYQDKSREYD), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PIK3R2

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Alternative Name: PIK3R2 (PI3K p85b Products)

Synonyms: MPPH antibody, P85B antibody, p85 antibody, p85-BETA antibody, pik3r2 antibody, MGC80449 antibody, p85beta antibody, phosphoinositide-3-kinase regulatory subunit 2 antibody, phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta) antibody, phosphoinositide-3-kinase, regulatory subunit 2 (beta) antibody, PIK3R2 antibody, pik3r2 antibody, Pik3r2 antibody

Background: PIK3R2 (Phosphatidylinositol 3-kinase, regulatory subunit 2), also called p85-Beta, is an enzyme that in humans is encoded by the PIK3R2 gene. The PIK3R2 gene is mapped on 19p13.11. Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene.

Synonyms: p85 antibody|p85 beta antibody|P85B antibody|P85B_HUMAN antibody|Phosphatidylinositol 3 kinase antibody|Phosphatidylinositol 3 kinase regulatory beta subunit antibody|Phosphatidylinositol 3 kinase regulatory subunit beta antibody|Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody|Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2(p85 beta) antibody|Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody|Phosphatidylinositol 3-kinase regulatory subunit beta antibody|Phosphoinositide 3 kinase regulatory subunit 2(beta) antibody|Phosphoinositide 3 kinase regulatory subunit 2 antibody|Phosphoinositide 3 kinase regulatory subunit polypeptide 2(p85 beta) antibody|Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody|Phosphoinositide 3 kinase, regulatory subunit 2(beta) antibody|Phosphoinositide 3 kinase, regulatory subunit 2(p85 beta) antibody|PI3 kinase p85 beta subunit antibody|PI3 kinase p85 subunit beta antibody|PI3-kinase regulatory subunit beta antibody|PI3-kinase subunit p85-beta antibody|PI3K antibody|PI3K regulatory subunit beta antibody|PIK3R 2 antibody|PIK3R2 antibody|PtdIns 3 kinase p85 beta antibody|PtdIns-3-kinase regulatory subunit beta antibody|PtdIns-3-kinase regulatory subunit p85-beta antibody

UniProt: O00459

Pathways: VEGF Signaling, BCR Signaling