P-Selectin antibody (N-Term)

Catalog No. ABIN3044277

Product Details anti-P-Selectin Antibody

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Binding Specificity: AA 49-66, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P-Selectin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for P-selectin(SELP) detection. Tested with WB, IHC-P in Human.

Sequence: KAYSWNISRK YCQNRYTD

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for P-selectin(SELP) detection. Tested with WB, IHC-P in Human.
Gene Name: selectin P(granule membrane protein 140 kDa, antigen CD62)
Protein Name: P-selectin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD62P(49-66aa KAYSWNISRKYCQNRYTD).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: SELP (SELP Products)

Synonyms: PSELECT antibody, CD62 antibody, CD62P antibody, GMP140 antibody, GRMP antibody, LECAM3 antibody, PADGEM antibody, PSEL antibody, GMP-140 antibody, Grmp antibody, selectin P antibody, selectin, platelet antibody, Selp antibody, SELP antibody

Background: P-Selectin, also called CD62, GRMP, GMP140 is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. The P-Selectin gene is mapped on 1q24.2. P-selectin plays an essential role in the initial recruitment of leukocytes(white blood cells) to the site of injury during inflammation. P-selectin is also very important in the recruitment and aggregation of platelets at areas of vascular injury. In quiescent platelet, P-selectin is located on the inner wall of alpha-granules. Sorting nexin-17 interacts with the cytosolic domain of P-selectin and suggested that SNX17 may function in the intracellular trafficking of P-selectin. P-selectin expression was found to be significantly and positively correlated with carotid artery stiffness and wall thickness, and the percentage of P-selectin-positive platelets was significantly higher in patients with carotid plaque.

Synonyms: Antigen CD62 antibody|CD62 antibody|CD62 antigen-like family member P antibody|CD62P antibody|CD62P antigen antibody|FLJ45155 antibody|GMP 140 antibody|GMP-140 antibody|GMP140 antibody|GMRP antibody|Granule membrane protein 140 antibody|Granule membrane protein 140 kDa antibody|Granulocyte membrane protein antibody|GRMP antibody|LECAM 3 antibody|LECAM3 antibody|Leukocyte endothelial cell adhesion molecule 3 antibody|Leukocyte-endothelial cell adhesion molecule 3 antibody|LYAM3_HUMAN antibody|P Selectin antibody|P-selectin antibody|PADGEM antibody|Platelet activation dependent granule external membrane protein antibody|Platelet activation dependent granule-external membrane protein antibody|Platelet alpha granule membrane protein antibody|PSEL antibody|Selectin P(granule membrane protein 140 kDa antigen CD62) antibody|Selectin P(granule membrane protein 140 kDa, antigen CD62) antibody|SELP antibody

UniProt: P16109