VEGFR2/CD309 antibody (Middle Region)

Catalog No. ABIN3044301

Cited in 6+ publications. The Rabbit Polyclonal anti-VEGFR2/CD309 antibody (ABIN3044301) specifically detects VEGFR2/CD309 in WB, IHC and ICC. The antibody is reactive with Human samples.

Quick Overview for VEGFR2/CD309 antibody (Middle Region) (ABIN3044301)

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFR2/CD309 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-VEGFR2/CD309 Antibody

Binding Specificity: Middle Region

Purpose: Anti-VEGF Receptor 2/KDR Antibody

Sequence: HHIHWYWQLE EECANE

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-VEGF Receptor 2/KDR Antibody. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human VEGF Receptor 2.

Isotype: IgG

Application Details

Application Notes: Immunocytochemistry , 0.5-1 μg/mL, Human, -Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, HumanWestern blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-VEGFR2/CD309 antibody (ABIN3044301)

Huang, Li, Pan, Cheng, Ren, Jia, Ma, Xu: "A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis." in: Oncotarget, Vol. 7, Issue 36, pp. 57705-57713, (2018) (PubMed).

Liu, Yang, Zhang, Shui, Song, Yao, Dai, Sun: "Fructopyrano-(1?4)-glucopyranose inhibits the proliferation of liver cancer cells and angiogenesis in a VEGF/VEGFR dependent manner." in: International journal of clinical and experimental medicine, Vol. 7, Issue 11, pp. 3859-69, (2014) (PubMed).

Zhu, Tuerxun, Hui, Abliz: "Effects of propranolol and isoproterenol on infantile hemangioma endothelial cells in vitro." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 647-651, (2014) (PubMed).

Wang, Liu, Wang, Wang: "Correlation of the expression of vascular endothelial growth factor and its receptors with microvessel density in ovarian cancer." in: Oncology letters, Vol. 6, Issue 1, pp. 175-180, (2013) (PubMed).

Wu, You, Ma, Li, Yuan, Li, Ye, Liu, Yao, Chen, Lai, Yang: "Role of transient receptor potential ion channels and evoked levels of neuropeptides in a formaldehyde-induced model of asthma in BALB/c mice." in: PLoS ONE, Vol. 8, Issue 5, pp. e62827, (2013) (PubMed).

Sun, Chen, Song, Ren, Wei, Liu, Zhang, Lv: "Effect of visfatin on the function of endothelial progenitor cells in high-fat-fed obese rats and investigation of its mechanism of action." in: International journal of molecular medicine, Vol. 30, Issue 3, pp. 622-8, (2012) (PubMed).

Target Details for VEGFR2/CD309

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Alternative Name: KDR

Background:

Background: KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Sait et al. (1995) likewise corrected the assignment to chromosome 4q11-q12 to the same region occupied also by PDGFRA and KIT, thus indicating the location of a cluster of receptor tyrosine kinase genes. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells (Plate et al., 1993). Flk1 is the mouse homolog of KDR (Matthews et al., 1991).

Gene Full Name: kinase insert domain receptor

Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Molecular Weight: 80 kDa

Gene ID: 3791

UniProt: P35968

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling