MMP16 antibody (C-Term)

Catalog No. ABIN3044303

Product Details anti-MMP16 Antibody

Target: MMP16 (Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16))

Binding Specificity: AA 582-598, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Matrix metalloproteinase-16(MMP16) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Sequence: YTVFQFKRKG TPRHILY

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Matrix metalloproteinase-16(MMP16) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: matrix metallopeptidase 16(membrane-inserted)
Protein Name: Matrix metalloproteinase-16(MMP-16)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MMP16(582-598aa, YTVFQFKRKGTPRHILY), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-MMP16 antibody (ABIN3044303)

Xue, Wu, Chen, Luo: "MT3-MMP down-regulation promotes tumorigenesis and correlates to poor prognosis in esophageal squamous cell carcinoma." in: Cancer medicine, Vol. 5, Issue 9, pp. 2459-68, (2017) (PubMed).

Wang, Li, Wu, Wu, Li, Yang, Chen, Xie, Huang, Du, Zhou: "miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro." in: OncoTargets and therapy, Vol. 8, pp. 3211-8, (2015) (PubMed).

Lin, Wang, Jie, Hong, Li, Wang, Yu: "Inhibitory effects of miR-146b-5p on cell migration and invasion of pancreatic cancer by targeting MMP16." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 31, Issue 4, pp. 509-14, (2011) (PubMed).

Target Details for MMP16

Target: MMP16 (Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16))

Alternative Name: MMP16 (MMP16 Products)

Synonyms: MT3-MMP antibody, Mt3mmp antibody, Mt3-mmp antibody, C8orf57 antibody, MMP-X2 antibody, MT-MMP2 antibody, MT-MMP3 antibody, matrix metallopeptidase 16 antibody, Mmp16 antibody, MMP16 antibody

Background: The matrix metalloproteinase 16(MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14 , MMP15 , and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms. MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle.

Synonyms: C8orf57 antibody|DKFZp761D112 antibody|Matrix metallopeptidase 16 membrane inserted antibody|Matrix metalloproteinase 16 antibody|Matrix metalloproteinase-16 antibody|Membrane type 3 matrix metalloproteinase antibody|Membrane type matrix metalloproteinase 3 antibody|Membrane-type matrix metalloproteinase 3 antibody|Membrane-type-3 matrix metalloproteinase antibody|MMP 16 antibody|MMP X2 antibody|MMP-16 antibody|MMP-X2 antibody|MMP16 antibody|MMP16_HUMAN antibody|MMPX2 antibody|MT MMP2 antibody|MT MMP3 antibody|MT-MMP 3 antibody|MT-MMP2 antibody|MT-MMP3 antibody|MT3 MMP antibody|MT3-MMP antibody|MT3MMP antibody|MTMMP2 antibody|MTMMP3 antibody|Putative transmembrane protein C8orf57 antibody

UniProt: P51512