BAFF antibody (C-Term)

Catalog No. ABIN3044436

Product Details anti-BAFF Antibody

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Binding Specificity: AA 265-285, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAFF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 13B(TNFSF13B) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: RENAQISLDG DVTFFGALKL L

Cross-Reactivity (Details): Predicted Cross Reactivity: rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 13B(TNFSF13B) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: tumor necrosis factor(ligand) superfamily, member 13b
Protein Name: Tumor necrosis factor ligand superfamily member 13B

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human BAFF(265-285aa RENAQISLDGDVTFFGALKLL), different from the related rat and mouse sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BAFF

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Alternative Name: TNFSF13B (TNFSF13B Products)

Synonyms: BAFF antibody, BLYS antibody, CD257 antibody, DTL antibody, TALL-1 antibody, TALL1 antibody, THANK antibody, TNFSF20 antibody, ZTNF4 antibody, BAFF-R antibody, BAFFR antibody, BROMIX antibody, CD268 antibody, CVID4 antibody, prolixin antibody, TNFSF13B antibody, RGD1561519 antibody, RGD1560810 antibody, BLyS antibody, D8Ertd387e antibody, zTNF4 antibody, 2010006P15Rik antibody, Baffr antibody, Bcmd antibody, Bcmd-1 antibody, Bcmd1 antibody, Lvis22 antibody, zgc:172115 antibody, TNF superfamily member 13b antibody, TNF receptor superfamily member 13C antibody, tumor necrosis factor (ligand) superfamily, member 13b antibody, tumor necrosis factor receptor superfamily, member 13c antibody, tumor necrosis factor superfamily member 13b antibody, TNFSF13B antibody, TNFRSF13C antibody, Tnfsf13b antibody, Tnfrsf13c antibody, tnfsf13b antibody

Background: BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system(CNS) lymphomas. The TNF superfamily member B cell-activating factor(BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology. The B cell activating factor BAFF(BlyS/TALL-1/zTNF4) is a tumor necrosis factor(TNF)-related ligand that promotes B cell survival and binds to three receptors(BCMA, TACI, and the recently described BAFF-R). Human BAFF was mapped to chromosome 13q32-34.4. The standard used in this kit is recombinant soluble human BAFF(A134-L295) with the molecular mass of 19.6KDa.

Synonyms: ApoL related ligand TALL 1 antibody|B cell Activating Factor antibody|B cell Activating Factor antibody|B lymphocyte stimulator antibody|B-cell-activating factor antibody|BAFF antibody|BLYS antibody|CD 257 antibody|CD257 antibody|CD257 antigen antibody|delta BAFF antibody|Dendritic cell derived TNF like molecule antibody|Dendritic cell-derived TNF-like molecule antibody|DTL antibody|DTL precursor antibody|PRO738 antibody|soluble form antibody|TALL 1 antibody|TALL-1 antibody|TALL1 antibody|THANK antibody|TN13B_HUMAN antibody|TNF and APOL related leukocyte expressed ligand 1 antibody|TNF homolog that activates apoptosis antibody|TNF homolog that activates apoptosis NKFB and JNK. antibody|TNF- and APOL-related leukocyte expressed ligand 1 antibody|TNFSF13B antibody|TNFSF20 antibody|Tumor necrosis factor ligand superfamily member 13B antibody|Tumor necrosis factor ligand superfamily member 20 antibody|Tumor necrosis factor like protein ZTNF4 antibody|Tumor necrosis factor superfamily member 13B antibody|UNQ401 antibody|ZTNF4 antibody

UniProt: Q9Y275

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response