NK-1.1/CD161c antibody (FITC)

Catalog No. ABIN1112510

Product Details anti-NK-1.1/CD161c Antibody (FITC)

Target: NK-1.1/CD161c (NK-1.1)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NK-1.1/CD161c antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Immunogen: NK-1+ cells from mouse spleen and bone marrow Species

Clone: PEK136

Isotype: IgG2a

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using 0,25 µg/10^6 cells

Comment:

Fluorescein Isothiocyanate, FITC (Abs/Em: 495/ 517 nm).

Reagent Preparation:

1. Transfer the sample to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse CD161 FITC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for NK-1.1/CD161c

Target: NK-1.1/CD161c (NK-1.1)

Abstract: NK-1.1/CD161c Products

Synonyms: AI462337 antibody, CD161 antibody, Ly-59 antibody, Ly55c antibody, Ly59 antibody, NK-RP1 antibody, NK1.1 antibody, NKR-P1.9 antibody, NKRP1 antibody, NKRP140 antibody, Nk-1 antibody, Nk-1.2 antibody, Nk1 antibody, Nk1.2 antibody, Nkrp1c antibody, ly-55c antibody, killer cell lectin-like receptor subfamily B member 1C antibody, Klrb1c antibody

Background: NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.