Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian , , and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
Purification
Protein G purified
Immunogen
PAX6 transcription factor linked to histidine tag.
WB, ELISA, IP
Host: Mouse
Monoclonal
33D10-D2
Biotin
Application Notes
Approved: Flo (1:10), IHC
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
PBS, 0.09 % sodium azide, 1 % BSA.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Product is photosensitive and should be protected from light. Avoid freeze-thaw cycles.
Storage
4 °C,-20 °C
Storage Comment
Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.