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His Tag antibody (FITC)

Name: His Tag antibody (FITC)
SKU: ABIN304882
Price: 667.7 USD
Availability: InStock

IHC, FACS Host: Mouse Monoclonal AD1-1-10 FITC

Image no. 1 for anti-His Tag antibody (FITC) (ABIN304882) (His Tag antibody (FITC))

1 image

Catalog No. ABIN304882

Datasheet as PDF

Target See all His Tag Antibodies

His Tag

Reactivity

Please inquire
Host
85

53

11

6

5

4

1

1
Mouse
Compatible Secondaries
Clonality
86

67

10
Monoclonal
Conjugate
71

22

13

11

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

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This His Tag antibody is conjugated to FITC
Application
132

85

34

25

23

14

14

14

13

13

12

7

6

6

5

4

4

4

3

3

2

2

1

1

1
Immunohistochemistry (IHC), Flow Cytometry (FACS)

Sequence

HHHHHH

Specificity

Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian , , and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.

Purification

Protein G purified

Immunogen

PAX6 transcription factor linked to histidine tag.

Type of Immunogen: Fusion protein

Clone

AD1-1-10

Isotype

IgG1

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Application Notes

Approved: Flo (1:10), IHC

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS, 0.09 % sodium azide, 1 % BSA.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Product is photosensitive and should be protected from light. Avoid freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.
Target

His Tag

Abstract

His Tag Products

Target Type

Tag