ErbB2/Her2 antibody (AA 1243-1255)

Catalog No. ABIN305740

Product Details anti-ErbB2/Her2 Antibody

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Binding Specificity: AA 1243-1255

Reactivity: Human, Mouse, Rat, Dog, Monkey, Horse, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: IHC-plus™

Specificity: Recognizes c-erbB-2 (185kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%) Elephant, Bat, Bovine (92%) Opossum (85%).

Purification: Protein A purified

Immunogen: A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%), Elephant, Bat, Bovine (92%), Opossum (85%).

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL detects c-erbB-2 in RIPA lysates from A431, MDA, MCF-7, LNCap, and c-erbB-2 transfected 3T3/NIH cells. Immunoprecipitation: 4 μg immunoprecipitated c-erbB-2 from 500 μg of A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: ERBB2 / HER2 (ErbB2/Her2 Products)

Synonyms: CD340 antibody, HER-2 antibody, HER-2/neu antibody, HER2 antibody, MLN 19 antibody, NEU antibody, NGL antibody, TKR1 antibody, Erbb-2 antibody, Neu antibody, c-erbB2 antibody, c-neu antibody, mKIAA3023 antibody, wu:fv70f10 antibody, zgc:63601 antibody, erb2 antibody, erb-b2 receptor tyrosine kinase 2 antibody, ERBB2 antibody, Erbb2 antibody, erbb2 antibody

Background: Name/Gene ID: ERBB2
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1

Gene ID: 2064

UniProt: P04626

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development