Caspase 9 antibody

Catalog No. ABIN306797

The Mouse Monoclonal anti-Caspase 9 antibody is suitable to detect Caspase 9 in samples from Human. It has been validated for WB.

Quick Overview for Caspase 9 antibody (ABIN306797)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 9 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: LAAP6 96 2-22

Product Details anti-Caspase 9 Antibody

Purpose: Mouse anti Human Caspase-9

Cross-Reactivity: Human

Characteristics: Caspases are key effectors of programmed cell death. They are synthesized as inactive proenzymes which are activated by cleavage at a specific aspartate residue to form two subunits. These subunits are normally linked together by a linker which may be involved in the regulation of the different caspases. Caspase-9 is a member of the CED-3 family and bear high similarity to caspase-3. Procaspase-9 can be activated by either caspase-3 or granzyme B, although they cleave the proenzyme to different size subunits. Cleavage by granzyme B produces an active enzyme which is capable of cleaving PARP. Also, the ability of caspase-3 to activate caspase-9 seems to suggest that caspase-9 is further downstream of caspase-3 and may be involved in later changes in cells observed undergoing apoptosis.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-9 protein and mouse myeloma cells.

Isotype: IgG

Application Details

Application Notes: Detects proenzyme form of human Caspase-9 by Western blot analysis as well as one of the cleavage products after treatment with thapsigargin. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: Caspase-9

Background: Cysteine-requiring Aspartate Protease-9, iCE-like apoptotic protease 6,Apoptotic protease Mch-6, Apoptotic protease-activating factor 3

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity