FAK antibody (C-Term)

Catalog No. ABIN306813

This anti-FAK antibody is a Rabbit Polyclonal antibody detecting FAK in WB and IP. Suitable for Mouse.

Quick Overview for FAK antibody (C-Term) (ABIN306813)

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAK antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-FAK Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse

Characteristics: Focal Adhesion Kinase (FAK) is a 120 kDa tyrosine kinase which become phosphorylated in response to oncogene- and extracellular-matrix integrin-dependent stimulation. Chicken, mouse and human FAK has been isolated and the deduced amino acid sequences showed identity of over 90 % . It is expressed in embryonic stem cells, and ubiquitously throughout development, in all adult tissues examined and in many cell lines. The highest expression of FAK is seen in the brain, testes and in osteoclasts. It is distinct from other non-receptor protein tyrosine kinases in that its catalytic domain is flanked by amino- and carboxy-terminal domains of about 400 amino acids each. Also, it does not contain the Src homology 2 or 3 domains, which mediate protein-protein interaction, nor a myristylation site that anchors proteins to the membrane.

Purification: Ammonium Sulfate Precipitation

Immunogen: Bacterially expressed fusion protein consisting of the C-terminal 150 amino acids of the mouse FAK

Isotype: IgG

Application Details

Application Notes: Detect focal adhesion kinase by IP/Western analysis in BALB/c 3T3 fibroblasts1. Detects a 120 kDa band. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Alternative Name: Focal Adhesion Kinase (FAK)

UniProt: P34152

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling