Estrogen Receptor alpha antibody

Catalog No. ABIN306907

This Mouse Monoclonal antibody specifically detects Estrogen Receptor alpha in WB, IP, ChIP, GS and InhA. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Pig, Dog, Chicken, Hamster and Sheep.

Quick Overview for Estrogen Receptor alpha antibody (ABIN306907)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Pig, Dog, Chicken, Hamster, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Inhibition Assay (InhA)

Clone: TE111-5D11

Product Details anti-Estrogen Receptor alpha Antibody

Purpose: Mouse anti Human Estrogen Receptor

Cross-Reactivity: Chicken, Cow, Dog, Hamster, Human, Mouse, Pig, Rabbit, Rat, Sheep

Characteristics: The estrogen receptor (ER) gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Purification: Protein A/G Chromatography

Isotype: IgG1

Application Details

Application Notes: This antibody can be used gel supershift assays (neat concentration), immunoprecipitation on native and denatured lysate (2 μg/mg of protein lysate), Western blotting (1-2 μg/mL) and chromatin precipitation. Can also be used to block binding of estrogen to the estrogen receptor. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: Estrogen Receptor

UniProt: P11474

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly