Clusterin antibody
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- Target See all Clusterin (CLU) Antibodies
- Clusterin (CLU)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Clusterin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Recognizes human clusterin, alpha at 40kD, and precursor protein.
- Purification
- Protein G purified
- Immunogen
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Native clusterin purified from human serum.
Type of Immunogen: Purified protein - Isotype
- IgG1 kappa
- Top Product
- Discover our top product CLU Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL. Detects human clusterin a chain (40kD) in RIPA lysates from HeLa cells, human serum and MCF-7 cell lysates. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 40 %
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Aliquot to Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.
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- Target
- Clusterin (CLU)
- Alternative Name
- CLU / Clusterin (CLU Products)
- Synonyms
- LOC100136120 antibody, clu antibody, MGC53055 antibody, zgc:73352 antibody, xclu antibody, MGC89163 antibody, CLU antibody, APO-J antibody, APOJ antibody, CLI antibody, CLU1 antibody, CLU2 antibody, KUB1 antibody, NA1/NA2 antibody, SGP-2 antibody, SGP2 antibody, SP-40 antibody, TRPM-2 antibody, TRPM2 antibody, DAG antibody, RATTRPM2B antibody, SP40 antibody, TRPM2B antibody, Trpm2 antibody, Trpmb antibody, AI893575 antibody, ApoJ antibody, Cli antibody, D14Ucla3 antibody, Sgp-2 antibody, Sgp2 antibody, Sugp-2 antibody, GP80 antibody, gpIII antibody, 40 antibody, clusterin-1 antibody, clusterin L homeolog antibody, clusterin antibody, Clusterin antibody, LOC100136120 antibody, clu.L antibody, clu antibody, CLU antibody, clus antibody, Clu antibody, LOC101113728 antibody
- Background
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Name/Gene ID: CLU
Synonyms: CLU, APO-J, Apolipoprotein J, CLU1, Clusterin, Complement lysis inhibitor, CLI, AAG4, Aging-associated protein 4, Ku70-binding protein 1, KUB1, SGP2, APOJ, SGP-2, Sulfated glycoprotein 2, CLU2, Complement cytolysis inhibitor, NA1/NA2, SP-40 - Gene ID
- 1191
- UniProt
- P10909
- Pathways
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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