CD8 antibody

Catalog No. ABIN316755

Product Details anti-CD8 Antibody

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognizes the Human CD8 cell surface antigen, a 32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells. Clone 4B11 has been reported as being suitable for use in Western blotting. AM05530SU-N is recommended for this purpose.

Characteristics: Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain

Immunogen: Synthetic peptide derived from CD8 alpha chain sequence.

Clone: 4B11

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 1/20-1/40. Immunohistochemistry on paraffin sections: 1/20-1/40. This product requires antigenretrieval using heat treatment prior to staining of paraffin sections.1 mM EDTA pH 8.0 isrecommended for this purpose. Positive Control Tissue: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1 mL distilled water.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one monthor (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD8

Target: CD8

Alternative Name: CD8 (CD8 Products)

Synonyms: CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 925

UniProt: P01732