FLT3 antibody

Catalog No. ABIN317712

The Rabbit Polyclonal anti-FLT3 antibody (ABIN317712) specifically detects FLT3 in WB and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for FLT3 antibody (ABIN317712)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-FLT3 Antibody

Specificity: Flt3 antibody detects endogenous levels of Flt3 protein.(region surrounding Asp593)

Purification: Affinity-chromatography

Application Details

Application Notes: ELISA: 1/1000 - 1/: 10000. Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % mM sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FLT3

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: CD135 / FLT3

Background: CD135 is a tyrosine kinase receptor expressed on normal cells including CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. CD135 is also expressed on malignant hematopoietic cells including AML, ALL and CML BC. CD135, also known as FMS-like tyrosine kinase 3, FLT3, STK1, and Flk2, is a growth factor receptor that binds the FLT3 ligand to promote the growth and differentiation of primitive hematopoietic cells. The intracytoplasmic domain of CD135 is modified by phosphorylation and has been shown to interact with Grb2, SOCS1, VAV1, and Shc. In humans, expression of Flt3 is restricted to subsets of CD34 positive as well as CD34 negative normal bone marrow cells. In these cells, the level of expression of Flt3 is rather low. Most of the CD34 bright Flt3+ cells co-express CD117 at high levels. They may represent early cycling, but not quiescent stem cells. Flt3+ cells in the CD34lo and CD34- populations do not co-express CD117 Molecule and may represent B lymphoid precursors.Synonyms: FL cytokine receptor, STK1, Stem cell tyrosine kinase 1, Tyrosine-protein kinase receptor FLT3

Gene ID: 2322

NCBI Accession: NP_004110

UniProt: P36888

Pathways: RTK Signaling