GORASP2 antibody

Catalog No. ABIN317911

Product Details anti-GORASP2 Antibody

Target: GORASP2 (Golgi Reassembly Stacking Protein 2, 55kDa (GORASP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GORASP2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: GRASP55 antibody detects endogenous levels of GRASP55 protein.(region surrounding Leu215)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GORASP2

Target: GORASP2 (Golgi Reassembly Stacking Protein 2, 55kDa (GORASP2))

Alternative Name: GORASP2 / GOLPH6 (GORASP2 Products)

Synonyms: MGC53281 antibody, MGC132131 antibody, MGC73072 antibody, zgc:73072 antibody, DKFZp459O1715 antibody, 0610011A07Rik antibody, 2410043M02Rik antibody, 5730520M13Rik antibody, 9430094F20Rik antibody, AW552058 antibody, GOLPH2 antibody, GRASP55 antibody, GRS2 antibody, p59 antibody, Grs2 antibody, GOLPH6 antibody, golgi reassembly stacking protein 2 L homeolog antibody, golgi reassembly stacking protein 2 antibody, gorasp2.L antibody, gorasp2 antibody, GORASP2 antibody, CC1G_02936 antibody, Gorasp2 antibody

Background: The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface. The structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil protein, the golgins and other peripheral membrane components such as GRASP55 and GRASP65. GRASP55 (Golgi reassembly stacking protien or p59) is a component of the Golgi stacking machinery. GRASP55 is highly homologous to GRASP65 and contains two PDZ domains. GRASP55 is myristoylated and palmitoylated. Unlike GRASP65, GRASP55 does not have detectable binding with the vesicle docking protein GM130 and is located on the medial-Golgi rather than cis-Golgi. Both GRASP55 and GRASP65 function in the stacking of Golgi cisternae. The novel coiled-coil protein golgin 45 interacts with GRASP55 and the GTP form of Rab 2, suggesting that GRASP55 and golgin 45 form a Rab 2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure. ERK2 directly phosphorylates GRASP55, which is phosphorylated in mitotic cells, suggesting that mitogen-activated protein kinase kinase (MKK)/ERK pathway phosphorylates the Golgi during mitosis.Synonyms: GRS2, Golgi marker, Golgi phosphoprotein 6, Golgi reassembly-stacking protein 2, Golgi reassembly-stacking protein of 55 kDa, p59

Molecular Weight: approx. 47 kDa

Gene ID: 26003

NCBI Accession: NP_056345

UniProt: Q9H8Y8