Six5 antibody

Catalog No. ABIN317916

Product Details anti-Six5 Antibody

Target: Six5 (SIX5) (Homeobox protein SIX5 (SIX5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Six5 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Six5 antibody detects endogenous levels of Six5 protein.(region surrounding Leu237)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Six5

Target: Six5 (SIX5) (Homeobox protein SIX5 (SIX5))

Alternative Name: SIX5 (SIX5 Products)

Synonyms: Dmahp antibody, MDMAHP antibody, TrexBF antibody, BOR2 antibody, DMAHP antibody, sine oculis-related homeobox 5 antibody, SIX homeobox 5 antibody, Six5 antibody, SIX5 antibody

Background: Six5 (homeobox protein SIX5), also known as SIX5, BOR2 or DMAHP (DM locus-associated homeodomain protein), is a transcription factor that is expressed in various structures of the adult eye. Localized to the cytoplasm in early development and to the nucleus in the later stages of development,Six5 is involved in regulation of organogenesis and in maintenance of retinal formation. Six5 is able to bind the 5'-TCA[AG][AG]TTNC-3' DNA sequence found in the myogenin and IGFBP5 promoters and, through this binding, can control transcription of the associated mRNA. Six5 is regulated via association with DACH1 (dachshund homolog 1) and is co-activated by the EYA (eyes absent) proteins. Defects in the gene encoding Six5 are the cause of branchiooto- renal syndrome type 2 (BOR2), an autosomal disorder characterized by hearing loss, a deep overbite and myopia. Two isoforms exist due to alternative splicing events.Synonyms: DM locus-associated homeodomain protein, DMAHP, Homeobox protein SIX5, Sine oculis homeobox homolog 5

Molecular Weight: approx. 75 kDa

Gene ID: 147912

NCBI Accession: NP_787071

UniProt: Q8N196