BRAF antibody (pThr753)

Catalog No. ABIN3180438

The Rabbit Polyclonal anti-BRAF antibody has been validated for WB and ELISA. It is suitable to detect BRAF in samples from Human, Mouse and Rat.

Quick Overview for BRAF antibody (pThr753) (ABIN3180438)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BRAF Antibody

Binding Specificity: pThr753

Specificity: Phospho-Raf-B (T753) Polyclonal Antibody detects endogenous levels of Raf-B protein only when phosphorylated at T753.

Characteristics: Rabbit Polyclonal to Phospho-Raf-B (T753).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Raf-B around the phosphorylation site of T753.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, ELISA 1:5000,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: Raf-B

Molecular Weight: 84 kDa

Gene ID: 673

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy