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p21 antibody (pThr145)
The Rabbit Polyclonal anti-p21 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect p21 in samples from Human, Mouse and Rat.
Quick Overview for p21 antibody (pThr145) (ABIN318089)
Target
See all p21 (CDKN1A) Antibodies
p21 (CDKN1A)
(Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
Reactivity
All reactivities for p21 antibodies
Human, Mouse, Rat
Host
All hosts for p21 antibodies
Rabbit
Clonality
All clonalities for p21 antibodies
Polyclonal
Conjugate
All conjugates for p21 antibodies
This p21 antibody is un-conjugated
Application
All applications for p21 antibodies
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
Product Details anti-p21 Antibody
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Binding Specificity
All epitopes for p21 antibodies
pThr145
Purification
Affinity Chromatography using epitope-specific immunogen
Alternatives
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anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 21-100) antibody
CDKN1A
Reactivity: Human, Mouse, Rat, Sheep
WB, ELISA, FACS, IHC (p), IF (p), IF (cc), ICC, IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Details
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Application Notes
ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Concentration
1.0 mg/mL
Buffer
Phosphate buffered saline (PBS), pH ~7.2 0.05 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target Details for p21
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Target
p21 (CDKN1A)
(Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
Alternative Name
CDKN1A / p21WAF1
Background
Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1
Gene ID
1026
NCBI Accession
NP_000380
UniProt
P38936
Pathways
p53 Signaling , PI3K-Akt Signaling , Cell Division Cycle , AMPK Signaling , Fc-epsilon Receptor Signaling Pathway , EGFR Signaling Pathway , Neurotrophin Signaling Pathway , Mitotic G1-G1/S Phases , DNA Replication , Hepatitis C , Synthesis of DNA , Autophagy
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