EGF antibody (Epidermal Growth Factor) (AA 973-1201)

Details for Product anti-EGF Antibody No. ABIN3208388, Supplier: Log in to see
Antigen
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Alternatives
anti-Rat (Rattus) EGF antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 973-1201
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12
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Reactivity
Dog (Canine)
382
186
109
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17
9
4
2
2
1
1
1
Host
Rabbit
370
148
32
26
6
3
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
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29
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1
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1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
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Options
Supplier
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Immunogen EGF (Asn973-Leu1201)
Isotype IgG
Specificity The antibody is a rabbit polyclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Plasmids, Primers & others Plasmids, Primers & others EGF products on genomics-online (e.g. as negative or positive controls)
Antigen
Background Alternative Names: URG, Beta-Urogastrone, Pro-epidermal growth factor
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
Application Notes
  • Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only
Format Liquid
Concentration 200 μg/mL
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Epidermal Growth Factor (EGF) (AA 973-1201) antibody (ABIN3208388) anti-Epidermal Growth Factor (EGF) (AA 973-1201) antibody
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