GRIK1 antibody (Internal Region)

Catalog No. ABIN334410

This anti-GRIK1 antibody is a Goat Polyclonal antibody detecting GRIK1 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for GRIK1 antibody (Internal Region) (ABIN334410)

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GRIK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-GRIK1 Antibody

Binding Specificity: Internal Region

Purpose: GLuR5 / GRIK1

Sequence: QCKQTHPTNS TS

Specificity: This antibody is expected to recognise both reported isoforms according to NP_000821.1 and NP_783300.1.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence QCKQTHPTNSTS, from the internal region (near the C Terminus) of the protein sequence according to NP_000821.1, NP_783300.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 150 kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 104 kDa according to NP_000821.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GRIK1

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Alternative Name: GRIK1

Background: GLUR5, GRIK1, glutamate receptor, ionotropic, kainate 1, EAA3 , EEA3 , GLR5 , excitatory amino acid receptor 3

Gene ID: 2897, 14805, 29559

NCBI Accession: NP_000821, NP_783300

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity