IFNAR2 antibody (Internal Region)

Catalog No. ABIN335143

This Goat Polyclonal antibody specifically detects IFNAR2 in ELISA, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for IFNAR2 antibody (Internal Region) (ABIN335143)

Target: IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IFNAR2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-IFNAR2 Antibody

Binding Specificity: Internal Region

Purpose: IFNAR2

Sequence: SYDSPDYTDE SCT

Specificity: This antibody is expected to recognise all three reported isoforms (NP_000865.2 , NP_997467.1, NP_997468.1 )

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SYDSPDYTDESCT, from the internal region of the protein sequence according to NP_000865.2 , NP_997467.1, NP_997468.1 .

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate and Placenta. Recommended concentration: 5 μg/mL.

Western Blot: Preliminary experiments gave an approx 28 kDa band in Human Uterus lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 37.4

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IFNAR2

Target: IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))

Alternative Name: IFNAR2

Background: IFNAR2, interferon (alpha, beta and omega) receptor 2, IFN-R, IFN-alpha-REC, IFNABR, IFNARB

Gene ID: 3455

NCBI Accession: NP_000865, NP_997467, NP_997468

Pathways: JAK-STAT Signaling, Hepatitis C