MED15 antibody (Internal Region)

Catalog No. ABIN335156

The Goat Polyclonal anti-MED15 antibody has been validated for WB and ELISA. It is suitable to detect MED15 in samples from Human.

Quick Overview for MED15 antibody (Internal Region) (ABIN335156)

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MED15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-MED15 Antibody

Binding Specificity: Internal Region

Purpose: PCQAP / MED15

Sequence: PPQSQTQPLV SQAQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_001003891.1, NP_056973.2).

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PPQSQTQPLVSQAQ, from the internal region of the protein sequence according to NP_001003891.1, NP_056973.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 90 kDa band observed in Human Lung lysates (calculated MW of 86.8 kDa according to NP_001003891.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MED15

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Alternative Name: MED15

Background: MED15, mediator complex subunit 15, ARC105, CAG7A, CTG7A, DKFZp686A2214, DKFZp762B1216, FLJ42282, FLJ42935, PCQAP, TIG-1, TIG1, TNRC7, CTG repeat protein 7a, PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein, PC2-glutamin

Gene ID: 51586, 94112

NCBI Accession: NP_001003891, NP_056973

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha