GCH1 antibody (AA 150-161)

Catalog No. ABIN343002

Product Details anti-GCH1 Antibody

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Binding Specificity: AA 150-161

Reactivity: Human, Cow, Dog, Horse, Pig, Bat, Chicken, Monkey, Xenopus laevis

Host: Goat

Clonality: Polyclonal

Conjugate: This GCH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human GCH1. This antibody is expected to recognize all four isoforms (NP_000152.1 , NP_001019195.1 , NP_001019241.1 , NP_001019242.1)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Elephant, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Xenopus (100%) Gorilla, Mouse, Rat, Hamster, Smelt, Sea anemone (92%) Trout, Seabass, Stickleback, Pufferfish, Zebrafish, Nematode, Yeast, Trichoplax, Salpingoeca (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-GKVHIGYLPNKQ from an internal region of human GCH1 (NP_000152.1 , NP_001019195.1 , NP_001019241.1 , NP_001019242.1 ). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Elephant, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Xenopus (100%), Gorilla, Mouse, Rat, Hamster, Smelt, Sea anemone (92%), Trout, Seabass, Stickleback, Pufferfish, Zebrafish, Nematode, Yeast, Trichoplax, Salpingoeca (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:16000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx. 37 kDa band observed in Human Lymph Node, Thymus and Tonsil lysates. Customers experiments gave identical results in lysates of cell line HUVEC with antibodies raised against different parts of the same protein. (calculated MW of 27.9 kDa according to NP_000152.1). Recommended concentration: 1-3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GCH1

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Alternative Name: GTPCH1 / GCH1 (GCH1 Products)

Synonyms: DYT14 antibody, DYT5 antibody, DYT5a antibody, GCH antibody, GTP-CH-1 antibody, GTPCH1 antibody, HPABH4B antibody, GTP-CH antibody, GTPCH antibody, Gch antibody, MGC89622 antibody, gch1a antibody, wu:fc16b10 antibody, zgc:195269 antibody, GTP cyclohydrolase 1 antibody, GTP cyclohydrolase 1-like antibody, GCH1 antibody, Gch1 antibody, gch1 antibody, Weevi_1190 antibody, Halhy_0838 antibody, Sinme_1220 antibody, Sphch_1077 antibody, LOC100136794 antibody

Background: Name/Gene ID: GCH1

Synonyms: GCH1, DYT14, DYT5a, Dystonia 14, GTP cyclohydrolase 1, GTP cyclohydrolase I, GTPCH1, HPABH4B, GTP-CH-1, DYT5, GCH, GTP-CH-I

Gene ID: 2643

NCBI Accession: NP_000152, NP_001019195, NP_001019241, NP_001019242

UniProt: P30793