Erythrocyte Ankyrin antibody (AA 1971-1983)

Catalog No. ABIN343025

Product Details anti-Erythrocyte Ankyrin Antibody

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Binding Specificity: AA 1971-1983

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Cow, Monkey, Bat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Erythrocyte Ankyrin antibody is un-conjugated

Application: ELISA

Specificity: Human ANK1. This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%) Turkey (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QIVKRASLKRGKQ from the C-terminus of human ANK1 (NP_000028.3, NP_065208.2, NP_065209.2, NP_065210.2, NP_065211.2, NP_065213.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%), Turkey (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:128000)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments gave approx. 19-23 kD bands in Human Skeletal Muscle lysates after 0.01 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kD bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kD according to NP_065211.2. The 19-22 kD bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Erythrocyte Ankyrin

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Alternative Name: ANK1 / Ankyrin (ANK1 Products)

Synonyms: CHANK1 antibody, wu:fa09h06 antibody, zgc:101835 antibody, ank antibody, sph1 antibody, sph2 antibody, ANK1 antibody, ANK antibody, SPH1 antibody, SPH2 antibody, Ank-1 antibody, nb antibody, pale antibody, ankyrin 1 antibody, ankyrin 1, erythrocytic a antibody, ankyrin 1 L homeolog antibody, ankyrin 1, erythrocytic antibody, ankyrin 1, erythroid antibody, ANK1 antibody, ank1a antibody, ank1 antibody, ank1.L antibody, Ank1 antibody

Background: Name/Gene ID: ANK1

Synonyms: ANK1, ANK-1, Ankyrin-1, Ankyrin-R, Erythrocyte ankyrin, ANK, Ankyrin, Ankyrin 1, erythrocytic, SPH2, SPH1

Gene ID: 286

NCBI Accession: NP_000028, NP_065208, NP_065209, NP_065210, NP_065211, NP_065213

UniProt: P16157

Pathways: Synaptic Membrane