His Tag antibody

Catalog No. ABIN344288

The Rabbit Polyclonal anti-His Tag antibody is suitable to detect His Tag. It has been validated for WB, ELISA, IP and IF.

Quick Overview for His Tag antibody (ABIN344288)

Target: His Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-His Tag Antibody

Sequence: HHHHHH

Specificity: Recognizes His-tag only. Species cross-reactivity: Expected to cross-react with proteins tagged with His from all species.

Purification: Immunoaffinity purified

Immunogen: 6x His synthetic peptide (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:25), IF, IP (1:25), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1:1000. Immunoprecipitation: 1:25. immunofluorescence (IF/IC): 1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Target Details for His Tag

Target: His Tag

Target Type: Tag