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Choline Acetyltransferase antibody (AA 590-640)

CHAT Reactivity: Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN350213
  • Target See all Choline Acetyltransferase (CHAT) Antibodies
    Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
    Binding Specificity
    • 16
    • 15
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 590-640
    Reactivity
    • 71
    • 43
    • 38
    • 17
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Mouse, Rat
    Host
    • 65
    • 7
    • 5
    • 3
    • 3
    Rabbit
    Clonality
    • 67
    • 16
    Polyclonal
    Conjugate
    • 47
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Choline Acetyltransferase antibody is un-conjugated
    Application
    • 44
    • 24
    • 24
    • 24
    • 17
    • 15
    • 13
    • 13
    • 8
    • 6
    • 6
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Specificity
    Specific for ChAT.
    Cross-Reactivity (Details)
    May contain small amounts of intact IgG
    Purity
    > 90 % based on SDS-PAGE
    Immunogen
    A synthetic peptide from aa regio 590-640 of mouse ChAT conjugated to an immunogenic carrier protein was used as the antigen.
    Isotype
    IgG
    Top Product
    Discover our top product CHAT Primary Antibody
  • Application Notes
    A concentration of 10-50 µg/ml is recommended.
    The optimal concentration should be determined by the end user.
    Not yet tested in other applications.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
    Handling Advice
    Avoid freeze and thaw cycles.
    Storage
    4 °C/-20 °C
    Storage Comment
    Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
    Expiry Date
    12 months
  • Wang, Wu, Wu, Sun, Zhang, Deng, Walker, Qu, Chen, Liu, Han, Dai, Shields, Shields, Sengelaub, Jones, Smith, Xu: "Remodeling of lumbar motor circuitry remote to a thoracic spinal cord injury promotes locomotor recovery." in: eLife, Vol. 7, (2019) (PubMed).

  • Target
    Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
    Alternative Name
    ChAT (CHAT Products)
    Synonyms
    CHOACTASE antibody, CMS1A antibody, CMS1A2 antibody, ChoAcTase antibody, B230380D24Rik antibody, choline O-acetyltransferase antibody, choline O-acetyltransferase a antibody, choline acetyltransferase antibody, carnitine O-acetyltransferase antibody, CHAT antibody, chata antibody, Chat antibody, PGTG_00551 antibody, LOC100533322 antibody
    Background
    Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. Choline acetylase  catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
    Catalytic activity: Acetyl-CoA + choline = CoA + O-acetylcholine. Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2), also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive. Also known as: CHOACTase, choline acetylase, choline O-acetyltransferase, CMS1A, CMS1A2, B230380D24Rik.
    Pathways
    Skeletal Muscle Fiber Development
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