GFP antibody

Catalog No. ABIN350302

This Sheep Polyclonal antibody specifically detects GFP in WB and IHC. It exhibits reactivity toward Aequorea victoriaand has been mentioned in 1 publication.

Quick Overview for GFP antibody (ABIN350302)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Sheep

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GFP Antibody

Purpose: Sheep antibody to EGFP/GFP/Venus

Specificity: Specific for EGFP/GFP/Venus

Cross-Reactivity (Details): Any organism expressing EGFP/GFP/Venus.

Purification: IgG

Immunogen: Recombinant EGFP/GFP/Venus protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

References for anti-GFP antibody (ABIN350302)

Choi, Kim, Song, Cha, Cho, Son, Kim, Mook-Jung: "Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death." in: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Background: GFP is anaturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.