GFAP antibody

Catalog No. ABIN351523

This Mouse Monoclonal antibody specifically detects GFAP in WB, IHC, IF, IHC (p) and IP. It exhibits reactivity toward Human, Rat, Pig and Chicken.

Quick Overview for GFAP antibody (ABIN351523)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Pig, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-GFAP Antibody

Brand: IHC-plus™

Specificity: Recognizes porcine glial fibrillary acidic protein (GFAP) at 51-52kD. Species cross-reactivity: human, rat and chicken.

Purification: Protein G purified

Immunogen: Porcine spinal cord. Cellular Localization: Cytoplasmic.

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-P (1 - 2 μg/mL), IP (2 μg/tst), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1-2 μg/mL. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate (Use Protein G). Western Blot: 1-2 μg/mL for 2 hrs at RT. Positive control: Astrocytoma, IMR5 and brain cells.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Name/Gene ID: GFAP

Synonyms: GFAP

Gene ID: 2670

UniProt: P14136