hCG antibody

Catalog No. ABIN356287

Product Details anti-hCG Antibody

Target: hCG (Chorionic Gonadotropin (hCG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This hCG antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises Human Chorionic Gonadotropin (hCG).
Cellular Localization: Cytoplasmic.

Characteristics: Synonyms: Choriogonadotropin subunit beta, CGB, CGB3, beta hCG, hCG-beta

Purification: Purified

Immunogen: Purified beta hCG. Remarks: Molecular weight of antigen: 22 kDa

Clone: HCG205

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. Antigen retriever like citrate enhances the staining of FFPE tissue sections (Boil tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes at RT. Recommended Positive Control: Human placenta, choriocarcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Storethe antibody undiluted at 2-8 °Cfor one month or (in aliquots) at -28 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for hCG

Target: hCG (Chorionic Gonadotropin (hCG))

Abstract: hCG Products

Synonyms: CGB antibody, AW108023 antibody, HCG antibody, chorionic gonadotropin, beta polypeptide antibody, ubiquitin-like 3 antibody, chorionic gonadotropin beta subunit 5 antibody, CGB antibody, Ubl3 antibody, CGB5 antibody

Background: Human chorionic gonadotropin (hCG) is a oligosaccharide glycopeptide hormone (244 aa) produced in pregnancy that is made by the embryo soon after conception and later by the syncytiotrophoblast (part of the placenta). Its role is to prevent the disintegration of the corpus luteum of the ovary and thereby maintain progesterone production that is critical for a pregnancy in humans. hCG may have additional functions, for instance, it is thought that hCG affects the immune tolerance of the pregnancy. Early pregnancy testing, in general, is based on the detection or measurement of hCG. HCG is compose of two non-identical, non-covalently linked polypeptide chains as α and β-subunits. The α subunit of hCG is nearly identical to that of thyroid stimulating (TSH), FSH, and LH.A germ cell tumor which is + for Cytokeratin, PLAP, and hCG but negative for EMA and alpha feto protein is probably choriocarcinomaSynonyms: CGB, CGB3, Choriogonadotropin subunit beta, beta hCG, hCG-beta

Gene ID: 1082

UniProt: P01233