PCNA antibody

Catalog No. ABIN356318

The Mouse Monoclonal anti-PCNA antibody has been validated for WB, IF, IHC (p) and IHC (fro). It is suitable to detect PCNA in samples from Human, Mouse, Rat, Saccharomyces cerevisiae and Insect. There is 1 publication available.

Quick Overview for PCNA antibody (ABIN356318)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae, Insect

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: PC10

Product Details anti-PCNA Antibody

Specificity: This antibody is specific for PCNA. PC10 reacts specifically with a 36 kDa nuclear protein of the Proliferating Cell Nuclear Antigen (PCNA). PCNA is an excellent marker of proliferative cells in routinely processed tissue sections.
Cellular Localization: Nuclear.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat, Insect and Yeast

Characteristics: Synonyms: Cyclin, Proliferating Cell Nuclear Antigen

Purification: Purified

Immunogen: Recombinant rat PCNA protein. Remarks: Molecular weight of antigen: ~30 kDa.

Isotype: IgG2a

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/200, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retriever (boiling tissue in 10 mM citrate, pH 6.0 for 20 mins, followed by cooling at RT for 20 mins). Recommended Positive Control: Human tonsil, lymph node. Western Blot: 1/100-1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

References for anti-PCNA antibody (ABIN356318)

Oka, Ohno, Tsuchimoto, Sakumi, Furuichi, Nakabeppu: "Two distinct pathways of cell death triggered by oxidative damage to nuclear and mitochondrial DNAs." in: The EMBO journal, Vol. 27, Issue 2, pp. 421-32, (2008) (PubMed).

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle It is increased during late G1 phase and S phase of the cell cycle and declines during G2 and M phases.Synonyms: Cyclin, Proliferating Cell Nuclear Antigen

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy