alpha Fetoprotein antibody

Catalog No. ABIN356479

Product Details anti-alpha Fetoprotein Antibody

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: AFP is a useful marker for detection of numerous developmental defects (open neural tube defect), hepatocarcinoma and endodermal sinus tumor (germ cell and yolk sac). Traces of AFP are found in normal adult sera and in greater concentrations in maternal, fetal and amniotic fluid.
Cellular Localization: Cytoplasmic.

Purification: Purified

Immunogen: Purified human GFAP.

Isotype: IgG

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 usingstreptavidinapprox. biotin system or polymer system. Recommended positive control: Human fetal liver, tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.05 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Abstract: AFP Products

Synonyms: FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody

Background: Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP

Gene ID: 174

NCBI Accession: NP_001125

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process