This antibody labels myeloid cells, histocytes, granulocytes, macrophages and monocyes. It is helpful in detection of myeloid or monocytic nature of acute leukemia. Cellular Localization: Cytoplasmic.
Purification
Purified
Immunogen
Purified human Lysozyme from the urine of patients with monocytic leukemia.
Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 usingstreptavidinapprox. biotin system or polymer system. Recommended positive control: Human tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
2.0 mg/mL
Buffer
PBS, pH 7.4 containing 0.05 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Lysozyme is 129 amino acids, 14.4 kDa enzyme that hydrolyzes 1,4 beta linkage between N-acetylmuramic acid and N-acetyl glucosamine residue in peptiglycan and between N-acetyl glucosamine's residues in chitodextrins. It is found in secretions, tears, saliva and mucus. It is also found in Cytoplasmic granules of the polymorphonuclear neutrophils (PMN) and egg whites. It has also been reported in spleen, kidney, lung, WBC and plasma.Synonyms: 1, 4-beta-N-acetylmuramidase C, LYZ, LZM