PHLPP1 antibody (N-Term)

Catalog No. ABIN360194

The Rabbit Polyclonal anti-PHLPP1 antibody has been validated for WB and EIA. It is suitable to detect PHLPP1 in samples from Human.

Quick Overview for PHLPP1 antibody (N-Term) (ABIN360194)

Target: PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHLPP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PHLPP1 Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to PHLPP1.

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PHLPP1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PHLPP1

Target: PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))

Alternative Name: PHLPP1

Background: PHLPP1 is a protein phosphatase that specifically mediates dephosphorylation of 'Ser-473' of AKT1, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes.Synonyms: KIAA0606, PH domain leucine-rich repeat-containing protein phosphatase 1, PHLPP, PLEKHE1, Pleckstrin homology domain-containing family E member 1, SCOP, Suprachiasmatic nucleus circadian oscillatory protein

Gene ID: 23239, 9606

UniProt: O60346

Pathways: PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway