GCKR antibody (N-Term)

Catalog No. ABIN361187

The Goat Polyclonal anti-GCKR antibody has been validated for WB and ELISA. It is suitable to detect GCKR in samples from Rat and Mouse.

Quick Overview for GCKR antibody (N-Term) (ABIN361187)

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Reactivity: Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GCKR antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GCKR Antibody

Binding Specificity: N-Term

Purpose: GCKR

Sequence: KRFQHVIETP EPGK

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KRFQHVIETPEPGK -C, from the N Terminus of the protein sequence according to NP_001477.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Mouse and Rat Liver lysates (calculated MW of 68.7 kDa according to Human NP_001477.1 and of 68.9 kDa according to Rat NP_037252.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GCKR

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Alternative Name: GCKR

Background: GCKR, glucokinase (hexokinase 4) regulator, GKRP, glucokinase (hexokinase 4) regulatory protein, glucokinase regulator, glucokinase regulatory protein, hexokinase 4

Gene ID: 2646, 231103, 25658

NCBI Accession: NP_001477

Pathways: Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus