Fibrillarin antibody

Catalog No. ABIN361375

Product Details anti-Fibrillarin Antibody

Target: Fibrillarin (FBL)

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibrillarin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: Specific for the ~34kDa Fibrillarin /Nop1p protein. It is expected that it will work on other mammal tissues.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Protein G purified culture supernatant

Immunogen: Yeast nuclear preparations

Clone: 38F3

Isotype: IgG1

Application Details

Application Notes: Recommended Dilution: WB: 1:1,000 IF: 1:500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: total IgG fraction + 10 mM Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-Fibrillarin antibody (ABIN361375)

Vendramin, Verheyden, Ishikawa, Goedert, Nicolas, Saraf, Armaos, Delli Ponti, Izumikawa, Mestdagh, Lafontaine, Tartaglia, Takahashi, Marine, Leucci: "SAMMSON fosters cancer cell fitness by concertedly enhancing mitochondrial and cytosolic translation." in: Nature structural & molecular biology, Vol. 25, Issue 11, pp. 1035-1046, (2019) (PubMed).

Langhendries, Nicolas, Doumont, Goldman, Lafontaine: "The human box C/D snoRNAs U3 and U8 are required for pre-rRNA processing and tumorigenesis." in: Oncotarget, Vol. 7, Issue 37, pp. 59519-59534, (2018) (PubMed).

Target Details for Fibrillarin

Target: Fibrillarin (FBL)

Alternative Name: Fibrillarin (Nop1p) (FBL Products)

Background: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Molecular Weight: '34 kDa

Gene ID: 851548

UniProt: P15646

Pathways: Ribonucleoside Biosynthetic Process