MAPK1/2 (pThr202), (pTyr204) antibody

Catalog No. ABIN361504

Product Details

Target: MAPK1/2

Binding Specificity: pThr202, pTyr204

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204 in Western Blots of UV treated Jurkat Cells or in a rat brain lysate. Immunolabeling is blocked by the Thr202/Tyr204 phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Human, Mouse, Rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: bovine, canine, chicken, mouse, non-human primates, Xenopus, zebra fish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References

Erickson, Ray, Wink, Bayon, Pedapati, Shaffer, Schaefer, Lahiri: "Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism." in: Journal of psychiatric research, Vol. 84, pp. 153-160, (2017) (PubMed).

Target Details

Target: MAPK1/2

Molecular Weight: 42/44 kDa

Gene ID: 116590

UniProt: P63086