MAPT antibody (Microtubule-Associated Protein tau) (pThr205)

Details for Product anti-MAPT Antibody No. ABIN362074, Supplier: Log in to see
Antigen
  • DDPAC
  • FTDP-17
  • MAPTL
  • MSTD
  • MTBT1
  • MTBT2
  • PPND
  • TAU
  • AI413597
  • AW045860
  • Mtapt
  • Tau
  • RNPTAU
  • pTau
  • tau
  • xtp
  • MAPT
  • PHF-tau
  • slc6a6
  • taut
  • wu:fc26e12
  • microtubule associated protein tau
  • microtubule-associated protein tau
  • microtubule associated protein tau S homeolog
  • Microtubule-associated protein tau
  • solute carrier family 6 (neurotransmitter transporter), member 6b
  • MAPT
  • Mapt
  • mapt.S
  • LOC5580230
  • CpipJ_CPIJ013260
  • tau
  • slc6a6b
Alternatives
anti-Human MAPT antibody for Western Blotting
Epitope
pThr205
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This MAPT antibody is un-conjugated
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Application
Immunohistochemistry (IHC), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen Peptide sequence around phosphorylation site of pThr205 (P-G-T (p) -P-G) derived from Human Tau. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of Tau only when phosphorylated at threonine 205.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Plasmids, Primers & others Plasmids, Primers & others MAPT products on genomics-online (e.g. as negative or positive controls)
Alternative Name Tau (MAPT Antibody Abstract)
Background Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Molecular Weight 78 kDa
UniProt P10636
Pathways MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Sun, Wei, Xiong, Wang, Xie, Wang, Yang, Wang, Lu, Liu, Wang: "Synaptic released zinc promotes tau hyperphosphorylation by inhibition of protein phosphatase 2A (PP2A)." in: The Journal of biological chemistry, Vol. 287, Issue 14, pp. 11174-82, 2012 (PubMed).

Chen, Xiong, Kong, Qu, Wang, Chen, Wang, Zhu: "Neuroglobin attenuates Alzheimer-like tau hyperphosphorylation by activating Akt signaling." in: Journal of neurochemistry, Vol. 120, Issue 1, pp. 157-64, 2011 (PubMed).

Tan, Cao, Wang, Lv, Wu, Ma: "Tau hyperphosphorylation is associated with memory impairment after exposure to 1.5% isoflurane without temperature maintenance in rats." in: European journal of anaesthesiology, Vol. 27, Issue 9, pp. 835-41, 2010 (PubMed).

Tan, Cao, Wang, Lv, Wu, Ma: "Protective effects of lithium treatment for spatial memory deficits induced by tau hyperphosphorylation in splenectomized rats." in: Clinical and experimental pharmacology & physiology, Vol. 37, Issue 10, pp. 1010-5, 2010 (PubMed).

Background publications Alonso, Mederlyova, Novak, Grundke-Iqbal, Iqbal: "Promotion of hyperphosphorylation by frontotemporal dementia tau mutations." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34873-81, 2004 (PubMed).

Li, Yin, Kuret: "Casein kinase 1 delta phosphorylates tau and disrupts its binding to microtubules." in: The Journal of biological chemistry, Vol. 279, Issue 16, pp. 15938-45, 2004 (PubMed).

Giasson, Sampathu, Wilson, Vogelsberg-Ragaglia, Mushynski, Lee: "The environmental toxin arsenite induces tau hyperphosphorylation." in: Biochemistry, Vol. 41, Issue 51, pp. 15376-87, 2002 (PubMed).

Taniguchi, Kawamata, Mukai, Hasegawa, Isagawa, Yasuda, Hashimoto, Terashima, Nakai, Mori, Ono, Tanaka: "Phosphorylation of tau is regulated by PKN." in: The Journal of biological chemistry, Vol. 276, Issue 13, pp. 10025-31, 2001 (PubMed).

Ganesan, Bass: "Isolation of C-I and C-II activated lipoprotein lipases and protamine insensitive triglyceride lipase by heparin-sepharose affinity chromatography." in: FEBS letters, Vol. 53, Issue 1, pp. 1-4, 1975 (PubMed).

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