Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
The antibody detects endogenous level of SEK1/MKK4 only when phosphorylated at serine 80.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer80 (T-H-S (p) -I-E) derived from Human SEK1, MKK4. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
MAP2K4
Reactivity: Human, Mouse, Rat
WB, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Saha, Jiang, Yang, Zhu, Wang, Schimmer, Qiu, Chang: "Targeting p53 via JNK pathway: a novel role of RITA for apoptotic signaling in multiple myeloma." in: PLoS ONE, Vol. 7, Issue 1, pp. e30215, (2012) (PubMed).
Target
MAP2K4
(Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))