KIT antibody (pTyr721)

Catalog No. ABIN362294

This Rabbit Polyclonal antibody specifically detects KIT in WB. It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for KIT antibody (pTyr721) (ABIN362294)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KIT Antibody

Binding Specificity: pTyr721

Specificity: The antibody detects endogenous level of c-Kit only when phosphorylated at tyrosine 721.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr721 (N-E-Y (p) -M-D) derived from Human c-Kit. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-KIT antibody (ABIN362294)

Gao, Lin, Gao, Li, Wang, Yu: "MicroRNA-193b regulates c-Kit proto-oncogene and represses cell proliferation in acute myeloid leukemia." in: Leukemia research, Vol. 35, Issue 9, pp. 1226-32, (2011) (PubMed).

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: c-Kit

Background: This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)

Molecular Weight: 145 kDa

NCBI Accession: NP_000213

UniProt: P10721

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity